The 2-Minute Rule for Recommended Template Preparation

9. Only Use  Look At This Piece , It is critical to utilize high quality ethanol in the wash buffer and not denatured alcohol. Denatured alcohol contains chemicals such as isopropanol, methanol and benzene, and these chemicals will not dry from the silica membrane, therefore making their method into your DNA option.

10. Elute Your DNA With Hot Elution Buffer, Heating the elution buffer to 70C before applying your sample to the column will release more of the DNA from the membrane, resulting in greater yields. Enabling the buffer to rest on the column for 5 minutes prior to centrifugation can also assist to increase yield.

Small DNA Fragment Extraction Kit - IBI Scientific

现货-QIAEX II Gel Extraction Kit (150)

How QIAEX II Gel Extraction Kit - ChemicalBook can Save You Time, Stress, and Money.

It does not have to be the bottleneck for getting to the genuine work of revealing the protein or genotyping your DNA. With these basic pointers, gel extraction will be a walk in the park, sending you on your way to ligation success in no time! Initially published on November 12, 2007.

Share this to your network:.